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New Generation

Taking Rejuvenation To The Next Level

bio-regenerative fibrin used during SEGOVA program

Professor Ljubić’s team has developed innovations in the ovaries and testes regeneration programme / SEGOVA New Generation.

SEGOVA New Generation (NG) is the most complete available medical biotechnological programme for the rejuvenation and improvement of gonadal function. The new generation of the rejuvenation programme has evolved due to the implementation of the most recent findings and novel technologies in clinical practice.

SEGOVA NG implies a shortened procedure, which demands to spend only one day in the hospital. This approach is possible due to the application of specific operative techniques during tissue retransplantation and the introduction of novel scaffolds (autologous activated bio-regenerative fibrin) which maintain retransplantated tissue and stem cells at the desired place within the ovarian or testicular tissue. SEGOVA NG also provides more precise visual control of tissue and stem cell retransplantation procedures. Segova NG consists of:

  1. Activated autologous highly concentrated platelet growth factors (hcPRP)

  2. Activated autologous bio-regenerative fibrin (BRF)

  3. Mesenchymal bone marrow stem cells (bm mSC)

  4. Activated ovarian cortex (IVA) tissue


Patient's participation in metabolic optimization and specific training.

SEGOVA is a biological regenerative therapy which has been done in hundreds of patients with 15 % success.

 SEGOVA NG consists of the following:

Preparation - one or more days or weeks, including analyses of:

hormones, immunological status, infections, metabolism, vitamins, microelements and genetics.

Performing of Procedure - during the one-day stay in the hospital, general anaesthesia

  • Blood is drawn from the patient to isolate and concentrate autologous PRP; BRF and autologous thrombin (fully automatised closed system).

  • Biopsy of the iliac bone and aspiration of the bone marrow.

  • Separation and processing of mSC from the bone marrow aspirate concentrate (fully automatised closed system)  

  • Minimally invasive laparoscopic surgery with tangential ovarian cortex excision.

  • Administration of autologous haemostatics instead of classical destructive surgical techniques

  • Micro-fragmentation of ovarian tissue submerged in mediums with autologous activated PRP - IVA

  • Creation of subcortical tunnels for the IVA ovarian cortical tissue and mSC retransplantation.

  • Application of autologous activated BRF scaffold in the adequate subcortical tunnels.

  • Application of the micro fragmented IVA ovarian cortical tissue with mSC into the adequate subcortical spaces.

  • Closure of the tunnels and re-creating the ovarian surface with the activated autologous BRF.

  • Control of haemostasis

Discharge from the hospital in 1 or 2 days.

The additional activities – the Nutritionist for Metabolic Optimisation and the physiotherapist – Gonadal HIIT program.

Follow-up - monthly or annual ultrasound and laboratory follow-up, with suggestions or implementation of the advanced in vitro fertilisation techniques.

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