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Biological In Vitro Fertilization (Bio-IVF) is a procedure of in vitro fertilization that involves the use of biological therapies. Biological therapies are based on cells and their products, such as platelet growth factors in enriched plasma (PRP) bio-regenerative fibrin (BRF), and cells with regenerative potential, such as mesenchymal and hematopoietic stem cells (m/hSC).

Bio-IVF is intended for patients with multiple failures due to the lack/poor quality of eggs and/or sperm, fertilization failure, irregular embryo development, endometrial factors and dysfunctional implantation. Also for those with advanced reproductive age, premature ovarian insufficiency, or a poor response to ovarian stimulation.

Autologous activated hcPRP
Highly concentrated plasma enriched with platelets (hcPRP) is a blood fraction obtained through a specialized automated centrifugation process, containing 6 to 20 times higher platelet concentrations than whole blood. Local application of PRP in the ovary has a regenerative effect and stimulates follicular growth, oocyte development, and surrounding cells responsible for hormone production. Additionally, hcPRP has been found to promote the growth of inadequate endometrium.

Autologous activated Bio-regenerative Fibrin (BRF)
Bio-regenerative fibrin is an autologous product that is obtained from the blood plasma by multiple filtrations during which the proteins necessary for blood clotting are concentrated. Autologous BRF retains growth factors locally in the tissue and serves as a scaffold. These products play an important role in intercellular communication and modulation of the immune response, thus contributing to the regenerative effect, improving ovarian function and regulating hormonal status.
Cells with regenerative potential – Mesenchymal and Hematopoietic Stem cells (m/hSC)
Stem cells are undifferentiated cells that can self-renew and differentiate into different types of cells. In tissues with altered function, they interact with existing cells and tissues, improving the conditions for the development and maturation of eggs. Furthermore, m/hSC may also improve the implantation capacity of the endometrial receptivity, if injected into the sub-endometrial region.
In vitro maturation of eggs (IVM)
In vitro maturation of eggs is the process of taking immature eggs from small immature follicles or ovarian tissue and then maturing them in laboratory (in vitro) conditions.:
Bio-IVF is used in several programs in the IVF procedure:

  1. BIOS: Biological Ovarian Stimulation (hcPRP/BRF, FSH/LH, GH, hCG)

  2. BISER: Biological Endometrial Receptivity Stimulation (hcPRP/BRF, hCG)

  3. BISER-O: Biological Endometrial Receptivity and Ovarian Stimulation (Ovarian hcPRP/BRF, FSH/LH, hCG; Endometrium hcPRP/BRF, hCG)

  4. SEGOVA: Ovarian rejuvenation (hcPRP/BRF, m/hSC, In Vitro Ovarian Tissue Activation)

  5. BITES: Biological Testicular Stimulation (hcPRP/BRF, FSH)

  6. Bio IVM: In Vitro Maturation (hcPRP in media)

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