Dual SEGO represents the second level of ovarian rejuvenation therapy, introducing two types stem cells as a key component to further enhance and restore ovarian function. Positioned between 

Bio IVF (Level 1) and SEGOVA and Dual SEGOVA (Level 3), it offers a more advanced regenerative approach while maintaining a minimally invasive profile.

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Dual therapy combines mesenchymal (mSC) and hematopoietic (hSC) stem cells to maximize their synergistic effects. This approach enhances the benefits of each cell type while enabling the collection of a larger number of stem cells, with the option for cryopreservation and later therapeutic augmentation.

 

Active principles in Dual SEGO:

1. hc PRP - Activated autologous highly concentrated Platelet Rich Plasma
2. BRF - Activated autologous bio-regenerative fibrin (BRF)
3. mSC - mesenchymal Stem Cells

4, hSC - hematopoietic Stem Cells

5. TPE - Therapeutic Plasma Exchange
6. Metabolic and physical optimization - Patient's participation in metabolic optimization and specific training.

Dual SEGO procedure consists of the following:

1. Preparation:

• one or more days or weeks of preparation, including analyses of hormones, immunological status, infections, metabolism, vitamins, micro-elements and genetics.

 
2. Performing of Dual SEGO Procedure:

• one single day hospital, general anaesthesia

• blood is drawn from the patient to isolate and concentrate autologous PRP, BRF (fully automatized closed system) and autologous thrombin.

• Therapeutic Plasma Exchange (TPE) 

• Pelvic bone biopsy with bone marrow aspiration

• separation/processing of the mSC  (fully automatized closed system)

• separation/processing of the hSC  (fully automatized closed system)

• cryopreservation of the SC surplus (optional)

• creation of subcortical tunnels for the SC re-transplantation (ultrasound guided)

• application of autologous activated BRF scaffold in the adequate subcortical tunnels (ultrasound guided)

• application of the mSC and hSC and autologous activated hcPRP into the adequate subcortical spaces (ultrasound guided)

• closure of the tunnels with the activated autologous BRF (ultrasound guided)

• control of hemostasis

• discharge from the hospital the following day 

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3. The additional activities:

• the Nutritionist for Metabolic Optimization and the physiotherapist gonadal HIIT program.

 
4. Reproductive follow-up:

• regular ultrasound and laboratory follow-up​