Prof. Aleksandar Ljubic
Testicular hcPRP/BRF
Testicular administration of highly concentrated platelet-enriched plasma/bio-regenerative fibrin.
Infertility caused by impaired gonadal function is particularly challenging to treat since the most effective methods of treatment (IVF with ovarian stimulation) do not produce the desired results. This problem is particularly emphasised by the postponement of reproduction to later life when the aging processes of gonads contribute to less healing success.
Biological therapies are an innovative approach to treatment for all these patients to restore gonadal function and fertility. Biological therapies, based on cells and their products, are used by various cells such as highly concentrated platelets/leukocytes in enriched plasma (hcPRP, PLRP), bio-regenerative platelet-enriched fibrin (BRF) or leukocytes (L-BRF).
hcPRP
hcPRP is a fraction of blood obtained by a special automatised centrifugation process that contains 4 to 8 times the concentration of platelets compared to full blood. Platelets are blood elements that play a fundamental role in blood coagulation and the healing process of injured tissues. They contain numerous growth factors that support cell proliferation, improve vascularisation and exhibit an immunomodulatory effect. Bearing in mind that hcPRP platelets are concentrated multiple times, their effect is much more potent.
BRF
Autologous-activated hcPRP can be applied alone or in combination with autologous-activated BRF, which retains growth factors locally in the tissue and serves as a substrate. These products play an important role in intercellular communication and modulation of the immune response, thus contributing to the regenerative effect, improving testicular function and regulating hormonal status.
The main indication for these procedures is the failure of the function of the testicles. This technology is aimed to correct not only the number of spermatozoa but also their quality. The problem of cell quality is manifested by the appearance, function and capacity to fertilize the egg and produce good good-quality embryos.
In stimulating protocols, in addition to injecting hcP(L)RP and BRF, follicle-stimulating hormone (FSH) is used, and sometimes small doses of human choriogonadotropic hormone (hCG). These products play an important role in intercellular communication and modulation of the immune response, thus contributing to the regenerative effect, improving testicular function and regulating hormonal status.
The described treatment represents a unique combination of biological therapies and IVF techniques and significantly increases the possibility of obtaining quality cells, which is one of the most important prerequisites for successful fertilization and pregnancy. This procedure is carried out under conditions of analgosedation, under the control of ultrasound. It can be performed separately, and in addition, testicular application of hcPRP/BRF is one of the procedures within the framework of a gonadal rejuvenation program – SEGOVA MAN.